RNAi interference
The Development of in vivo RNAi technologies
The objective of these groups is to develop novel technologies for efficient RNAi in mammalian cells. The Martinez and Dickson groups will take biochemical and genetic approaches, respectively, to identify and characterize novel factors involved in the basic RNAi machinery. The Peters group will test whether these factors can be used to modulate the efficacy or specificity of RNAi in mammalian tissue culture cells. This work will lead to improved methods for RNAi that have been validated in mammalian cells in vitro. Such reagents and technology advantages can then be adapted by the mouse groups for RNAi in vivo.
